At present, there are many techniques for studying protein-protein interaction (PPI), each of which has its own advantages and disadvantages, and some methods for studying PPI have various limitations, especially in the study of dynamic PPI. A technology called bioluminescence resonance energy transfer (BRET) was developed about two decades ago. 	bioluminescence resonance energy transfer assays	https://www.iaanalysis.com/bioluminescence-resonance-energy-transfer-bret-platform.html